Expression Vectors

Expression Vectors - been able to remove the gall-forming genes and insert other genes into the plasmid As a result this plasmid has enabled

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Expression Vectors The promoter and ribosome binding site codes in Eukaryotic DNA are likely to be different than those used by the host organism. Plasmids called expression vectors have been created that have a promoter and ribosome binding site that can be recognized by E. coli . These sites are adjacent to a restriction cutting site so that any gene inserted into the plasmid will be transcribed and translated by E. coli . Bacterial Artificial Chromosomes Artificial vectors have been developed that can be used for inserting large segments of DNA. These vectors are called bacterial artificial chromosomes. Transgenic Plants The only plasmid that plant cells take up is the Ti (tumor-inducing) plasmid from the bacterium Agrobacterium. The plasmid transferred by this bacterium causes plants to form a gall. A wide variety of plant cells will take up the plasmid and move it into a chromosome. Scientists have
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Unformatted text preview: been able to remove the gall-forming genes and insert other genes into the plasmid. As a result, this plasmid has enabled genetic engineering of a large number of plants. Transgenic Animals Animal cells generally will not take up plasmids. Other methods such as microinjection must be used. One method has been developed where animal eggs are placed in a mixer with needle-like fragments of silicon carbide. The needles make holes in the cells, allowing DNA to enter. Using this procedure, eggs from fish and several agricultural species have been given the gene for bovine growth hormone, producing larger individuals. Artificial Chromosomes Artificial chromosomes have been created that serve as vectors for introducing large segments of DNA into eukaryotic cells. These chromosomes contain sequences that enable their replication in certain kinds of cells....
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This note was uploaded on 12/15/2011 for the course BIO BSC1010 taught by Professor Gwenhauner during the Fall '10 term at Broward College.

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