Making Recombinan t DNA To make recombinant DNA, restriction enzymes are used to cut DNA from two sources such as the that of a vector and a gene to be cloned. If the vector and the gene to be cloned are both cut with the same restriction enzyme, they will both have complimentary sticky ends (see above). After cutting, the two samples of DNA are mixed. Some of the fragments from one species will stick to those of the other because they both have the same sticky ends. DNA ligase is used to seal the fragments. Bacteria are capable of taking up DNA from their environment. This process is called transformation . CaCl 2 and a procedure called heat shock are used to make E. coli cells more permeable so that they take up the modified plasmids more readily. Genomic Libraries A genome is all of the genes in a particular organism. Bacteria or virus vectors can be used to store fragments of the DNA from a species. The DNA is cut up into fragments and the different fragments are inserted into bacteria or
This is the end of the preview. Sign up
access the rest of the document.
This note was uploaded on 12/15/2011 for the course BIO BSC1010 taught by Professor Gwenhauner during the Fall '10 term at Broward College.