Radioactive Probes

Radioactive Probes - Radioactive Probes The blue-white...

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Radioactive Probes The blue-white screening method described above selects for bacteria that have any gene. Radioactive probes can be used to find colonies that have specific genes. Probes are short, single-stranded segments of DNA whose base sequence matches part of the gene in question. It is not necessary to match the entire gene, just a small fragment. The cells are lysed and the DNA is denatured by treating it with an alkaline solution. When the probe is mixed with the denatured DNA, it will form a double-helix in the area where the gene has complimentary bases. If the probe is radioactive or fluorescent, it can be visualized. The gene can then be isolated or cloned as needed. It may be possible to see the chromosome and the location on the chromosome while viewing under a microscope. Autoradiography is a process in which film is used to show the area of the vector where the probe has attached. This area is the gene in question. Obtaining Probes
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Radioactive Probes - Radioactive Probes The blue-white...

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