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Unformatted text preview: has allowed much of the biotechnology development that we have seen in recent years. Materials and Procedure Materials needed The procedure requires primers, DNA polymerase, and nucleotides. Primers are short chains of about 20 nucleotides that are complimentary to a region in the DNA to be amplified. They are needed because the enzyme that copies the DNA (DNA polymerase) cannot start the process unless it has already been started. DNA polymerase from the thermophile Thermus aquaticus is used because this species thrives at temperatures that are near boiling. It's DNA polymerase (called Taq polymerase) is stable at relatively high temperatures and functions optimally at 70 degrees C. This is important because high temperatures will be used to separate the strands of the double helix. Nucleotides are needed because DNA is composed of nucleotide "building blocks"....
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