COOMBS TEST - COOMBS TEST Indirect Coombs test(Indirect...

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COOMBS TEST
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Indirect Coombs test (Indirect Antiglobulin test): This test is performed to detect presence of Rh-antibodies or other antibodies in patients serum in case of the following: 1. To check whether an Rh-negative women (married to Rh- positive husband) has developed Anti Rh-antibodies 2. Anti D may be produced in the blood of any Rh-negative person by exposure to D antigen by- Transfusion of Rh positive blood Pregnancy, if infant is Rh positive (if father is Rh-positive) Abortion of Rh-positive fetus.
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Requirements: Test tubes: (10x75 mm) Pasteur pipettes Incubator Centrifuge Specimen: Serum (need not be fasting) Reagents: 1. Antihuman serum 2. Anti-D serum Additional Requirements: Coombs control cells A. Make a pooled ‘O’ Rho (D) positive cells from at least three different ‘O’ positive blood samples. B. Wash these cells three times in normal saline (these cells should be completely free from serum with no free antibodies). Make 5 % saline suspension of these cells
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COOMBS TEST - COOMBS TEST Indirect Coombs test(Indirect...

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