1 Materials & Methods 3-1

1 Materials & Methods 3-1 - Molecular Biology...

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1 Molecular Biology Labels, Transfers, Probing, Chips
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2 Probing Southern blots: DNA Northern blots: RNA Western blots: protein All attach a labeled “probe” macromolecule to a specific target that has been transferred from an electrophoretic gel to a filter membrane
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3 Alberts et al. Molecular Biology of the Cell
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4 Alberts et al. Molecular Biology of the Cell ATP labeled in different locations with different isotopes
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5 Synthesizing a probe: dNTPs labeled Label can be alpha 32 P – or can be some kind of chemical tag
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6
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7 Alberts et al. Molecular Biology of the Cell
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8 Source: www.mun.ca/biology/scarr/ 3250_Recombinant_DNA.htm , who copied it from Watson et al. Recombinant DNA
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9 Genomic DNA digest with Eco RI, probed for mtDNA cytochrome oxidase subunit III gene size standards: λ DNA cut with Hind III DNA that didn’t digest (it happens)
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10 Right: lane 3 probed with labeled DNA Left: DNA digested ethidium bromide DNA size standards 1 2 3
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1 Materials & Methods 3-1 - Molecular Biology...

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