2 Transcription - Molecular Biology Part II: The Central...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Molecular Biology Part II: The Central Dogma Transcription
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 “DNA does nothing without proteins” How do we know when proteins interact with DNA? to interact, molecules have to stick together Some more M&M: three tests for proteins sticking to DNA 1. “filter binding assay” 2. “mobility shift assay” 3. “DNA footprint assay”
Background image of page 2
3 Weaver 2005 Molecular Biology 3rd ed. Nitrocellulose filter binds: - protein - ssDNA - not dsDNA DNA-protein binding assay #1: Filter-binding assay radiolabel DNA Filter retains radioactivity if protein binds to DNA protein
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 Weaver 2005 Molecular Biology 3 rd ed. DNA-protein binding assay #2 Indirect binding – second protein will shift DNA mobility only when first protein is present
Background image of page 4
5 Weaver 2005 Molecular Biology 3rd ed. Assay #3: DNAse footprinting locates protein binding
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
6 Overview 1. Initiation 2. Elongation 3. Termination
Background image of page 6
7 Lewin 1990 Genes IV Steps in the initiation of transcription Note startpoint of transcription is NOT the start point of translation!
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
8 RNA polymerase Holoenzyme = core + σ (sigma)
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 12/24/2011 for the course STEP 1 taught by Professor Dr.aslam during the Fall '11 term at Montgomery College.

Page1 / 27

2 Transcription - Molecular Biology Part II: The Central...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online