3 Regulation Operons2

3 Regulation Operons2 - Molecular Biology Part III...

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1 Molecular Biology Part III Regulation of Gene Expression B. Operons, Part 2
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3 The lac operon model: from structure to function The lac model: from structure to function How does lac work? How do we know? What does lac look like in 3-D? Another operon: ara (a BAD operon!)
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4 Weaver 2005 Molecular Biology 3rd ed. Fig 7.16 Map of the lac operon: defined by genetic mapping (e.g. interrupted mating and deletion analyses) and by footprinting. (n.b.: first published versions were wrong!)
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5 Weaver 2005 Molecular Biology 3rd ed. Fig 7.3
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6 Weaver 2005 Molecular Biology 3rd ed. Fig 7.5-a/b
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7 Weaver 2005 Molecular Biology 3rd ed. Fig 7.5-c/d
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8 Weaver 2005 Molecular Biology 3 rd ed. Fig 7.6 nitrocellulose filter binding assay: -- filter binds protein -- protein binds to radiolabled DNA
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9 Weaver 2005 Molecular Biology 3rd ed. Fig 7.7 More filter-binding assay of lac repressor function: binding to mutant operator wild-type operator mutant operator Conclusion: “operator” is DNA that works by binding to repressor protein
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10 crystal of lac repressor protein adding IPTG shatters crystal: indicates strong binding to repressor
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Weaver 2005
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This note was uploaded on 12/24/2011 for the course STEP 1 taught by Professor Dr.aslam during the Fall '11 term at Montgomery College.

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3 Regulation Operons2 - Molecular Biology Part III...

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