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Eukaryotes D - 15 • remember the “Flavr-Savr” tomato...

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1 Molecular Biology Part IV Eukaryotes: Regulation, Hyperdrive, & the C-Value Paradox D. more mRNA hijinks
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2 RNA editing trypanosomes guide RNA nucleotide deamination
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3 Weaver 2005 Molecular Biology 3rd ed.
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4 Lewin 1990 Genes IV
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5 Weaver 2005 Molecular Biology 3rd ed. CO III editing: 407 U’s added 19 U’s deleted
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6 Weaver 2005 Molecular Biology 3rd ed.
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7 Weaver 2005 Molecular Biology 3rd ed.
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8 Lewin 1990 Genes IV
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9 Lewin 2000 Genes VII
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10 who needs DNA?
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11 wild type flowers “hothead” mutant Arabidopsis thaliana hth / hth HTH / hth or HTH / HTH !! self-fertilization
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12 “Hothead”: genetic reversion in Arabidopsis : mutant alleles ( hth ) revert to wild-type (HTH) at extremely high frequencies, apparently directed by an unknown template Weigel & Jurgens, Lolle et al. 2005 Nature * Reversion
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13 Science Sept 2006 … or not! (oops!)
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14 still more RNA hijinks RNA silencing, RNA interference (RNAi)
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15 remember the “Flavr-Savr” tomato?
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Unformatted text preview: 15 • remember the “Flavr-Savr” tomato? No? 16 17 Caenorhabditis elegans embryos: in situ probing for mex-3 mRNA neg. control no mex-3 probe positive control with mex-3 probe Embryo from parent injected with mex-3 antisense RNA Embryo from parent injected with mex-3 dsRNA note mRNA remains and hybridizes with probe mex-3 mRNA is entirely absent ! dsRNA has resulted in RNA inter-ference th 18 RNAi • Fire et al. demonstrated that dsRNA caused mRNA suppression – only worked if dsRNA corresponded to exons, not to introns or promoters – dsRNA was able to cross cell boundaries and affect whole organism (at least in C. elegans ) 19 Readers’ Digest 20 Weaver 4th ed. 21 Weaver 4 th ed. 22 Weaver 4th ed. 23 www.ambion.com 24 25 26 miRNA 27 28 29 30 31 just what you always wanted…...
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