Mesenchymal stem cells 3.57.51 PM

Mesenchymal stem cells 3.57.51 PM - MESENCHYMAL STEM CELLS...

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MESENCHYMAL STEM CELLS SK Cheong, Dept of Diagnostic Laboratory Services, Hospital UKM
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WHAT ARE MESENCHYMAL STEM CELLS (MSC) ? First isolated by Friedenstein et al in 1974 Fibroblastoid cells - spindle-shaped Adherent to tissue culture glass or plastic High growth potential
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WHY ARE MSC INTERESTING ? Rat MSC genetically tagged are  microinjected into a mouse blastocyte Blastocyte reimplanted into another rat Blastocyte allowed to develop into baby  rat -> sacrificed Gene tags are found in many of the baby  rat tissues tested ->Embryonic stem cells?
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Mok & Cheong 2002
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In vitro MSC growth pattern- doubling time 33hr (Conget et al 1999)
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HOW TO ISOLATE MSC? Sources: BM, periosteum, synovium, fat,  cord blood, peripheral blood, foetal liver  and lung Adherent to culture bottle; haemopoietic  stem cells do not Easily subcultured
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Morphology Adherent to glass or plastic Surface markers: SH2, SH3, SH4, STRO- 1, ICAM-2, NCAM, integrins, PDGF, IL-IR
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Mesenchymal stem cells 3.57.51 PM - MESENCHYMAL STEM CELLS...

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