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EXAM2KEY

# EXAM2KEY - 1-b 2-b 3-c 4-c 5-b 6-d 7-a 8-c 9-c 10-d 11-e...

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BCH 3033 Exam 2 Key (Corrected) and Grade Distribution Form 1. Multiple Choice: 1-a 2-b 3-d 4-a 5-d 6-c 7-b 8-c 9-b 10-d 11-e 12-e 13-b 14-c 15-b 16-a 17-b 18-e 19-a 20-e 21-e 22-e 23-d 24-d 25-c 26-b 27-a 28-c 29-c 30-c Written Answers: 1. IRAVE needed to have regular peptide bonds (in trans) and all atoms showing. 2. a. You needed to calculate the total activity (converting activity of the 50μL sample to the total volume of the fraction). Then dividing by the total protein of each fraction. Crude Extract: 1.76 μmoles/sec/mg protein 20-40% cut: 4.43 μmoles/sec/mg protein Affinity Chrom: 139.3 μmoles/sec/mg protein b. “the last step” 139.3 μmoles/sec/mg protein / 4.43 μmoles/sec/mg protein = 31.4X c. 961 μmoles/sec total act recovered / 1380 μmoles/sec total act crude x 100 = 70 % Form 2. Multiple Choice:
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Unformatted text preview: 1-b 2-b 3-c 4-c 5-b 6-d 7-a 8-c 9-c 10-d 11-e 12-e 13-b 14-c 15-b 16-d 17-b 18-e 19-a 20-e 21-d 22-e 23-c 24-c 25-c 26-d 27-d 28-c 29-d 30-d 2. a. You needed to calculate the total activity (converting activity of the 50μL sample to the total volume of the fraction). Then dividing by the total protein of each fraction. Crude Extract: 2.01 μmoles/sec/mg protein 20-40% cut: 6.68 μmoles/sec/mg protein Affinity Chrom: 128.8 μmoles/sec/mg protein b. “the last step” 128.8 μmoles/sec/mg protein / 6.68 μmoles/sec/mg protein = 19.3X c. 1069 μmoles/sec total act recovered / 1984 μmoles/sec total act crude x 100 = 53.8% SCORING: Multiple Choice (2 x Test Score) + 10 + Written Score = Total Score Average: 46.7 +/- 12.4 High = 86 Low = 23...
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