EXAM2KEY

EXAM2KEY - 1-b 2-b 3-c 4-c 5-b 6-d 7-a 8-c 9-c 10-d 11-e...

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BCH 3033 Exam 2 Key (Corrected) and Grade Distribution Form 1. Multiple Choice: 1-a 2-b 3-d 4-a 5-d 6-c 7-b 8-c 9-b 10-d 11-e 12-e 13-b 14-c 15-b 16-a 17-b 18-e 19-a 20-e 21-e 22-e 23-d 24-d 25-c 26-b 27-a 28-c 29-c 30-c Written Answers: 1. IRAVE needed to have regular peptide bonds (in trans) and all atoms showing. 2. a. You needed to calculate the total activity (converting activity of the 50μL sample to the total volume of the fraction). Then dividing by the total protein of each fraction. Crude Extract: 1.76 μmoles/sec/mg protein 20-40% cut: 4.43 μmoles/sec/mg protein Affinity Chrom: 139.3 μmoles/sec/mg protein b. “the last step” 139.3 μmoles/sec/mg protein / 4.43 μmoles/sec/mg protein = 31.4X c. 961 μmoles/sec total act recovered / 1380 μmoles/sec total act crude x 100 = 70 % Form 2. Multiple Choice:
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Unformatted text preview: 1-b 2-b 3-c 4-c 5-b 6-d 7-a 8-c 9-c 10-d 11-e 12-e 13-b 14-c 15-b 16-d 17-b 18-e 19-a 20-e 21-d 22-e 23-c 24-c 25-c 26-d 27-d 28-c 29-d 30-d 2. a. You needed to calculate the total activity (converting activity of the 50L sample to the total volume of the fraction). Then dividing by the total protein of each fraction. Crude Extract: 2.01 moles/sec/mg protein 20-40% cut: 6.68 moles/sec/mg protein Affinity Chrom: 128.8 moles/sec/mg protein b. the last step 128.8 moles/sec/mg protein / 6.68 moles/sec/mg protein = 19.3X c. 1069 moles/sec total act recovered / 1984 moles/sec total act crude x 100 = 53.8% SCORING: Multiple Choice (2 x Test Score) + 10 + Written Score = Total Score Average: 46.7 +/- 12.4 High = 86 Low = 23...
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This note was uploaded on 12/26/2011 for the course BCH 3033 taught by Professor Makemson during the Fall '08 term at FIU.

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