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Unformatted text preview: ______________________________ __ _______________________________ Your name Your TA's name BioGD2810 Genetics Fall 2010 Quiz #8 The pathways for the biosynthesis of the amino acids glutamine (gln) and proline (pro) involve one or more common intermediates. Auxotrophic haploid yeast mutants numbered 1-7 are isolated that require either glutamine or proline or both amino acids for their growth. These mutants are also tested for their ability to grow on the intermediates A-E. The results are shown in the following table (+ means growth on minimal medium containing the indicated supplement(s); - means no growth observed). Mutant A B C D E gln pro gln+pro 1 +--- +- + + 2------ + + 3-- +---- + 4----- +- + 5-- + +--- + 6 +----- + + 7- +--- +- + ( a) (10 points) In the box below, indicate the order of all the intermediates and end products in the glutamine and proline pathways, and label the point at which the pathway is blocked in each mutant 1-7. [Note: this part of the problem is taken directly from the book.] The remaining parts of the problem do not depend on your answer to part a. Continued next page Additional haploid mutants 8, 9, and 10 in this pathway are found, and they all show the same pattern of growth see in mutant 7 above (the entry for mutant 7 is repeated for convenience): Mutant A B C D E gln pro gln+pro 7- +--- +- + 8- +--- +- + 9- +--- +- + 10- +--- +- + Haploid cells containing mutations 7-10 are mated to make diploid cells as shown in the table below. + means growth of the diploid on minimal medium; - means no growth.+ means growth of the diploid on minimal medium; - means no growth....
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This note was uploaded on 12/20/2011 for the course BIOGD 6057 taught by Professor Fox during the Spring '09 term at Cornell University (Engineering School).
- Spring '09