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Unformatted text preview: ______________________________ __ _______________________________ Your name Your TA's name BioGD2810 Genetics Fall 2010 Quiz #10 A cross is made between an Hfr E. coli strain of genotype gln + arg + oxycycline S R ampicillin R bacteriophage T7 R , and an F- strain of genotype gln- arg- oxycycline R S ampicillin S bacteriophage T7 S . (+ means prototrophic and means auxotrophic for the given amino acid [glutamine (gln) or arginine (arg)]; S means sensitive and R means resistant to the antibiotics oxycycline (oxy), (tet) or ampicillin (amp); or to bacteriophage T7). After mating, the cells are disrupted at various times and then plated on petri plates with complete (rich) medium containing oxycycline and . 36 colonies from these plates are chosen, and then replica plated on rich medium containing either bacteriophage T7 or ampicillin, on minimal medium, and on minimal medium with amino acid supplements (minimal + gln or minimal + arg). The results of this analysis are shown on the extra page; a dark circle means that a minimal + arg)....
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- Spring '09