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Unformatted text preview: base pairs on either side of the restriction site for cleavage to occur. [This is a very difficult problem, but it will help you review a substantial amount of material thus far encountered in the course.] 5' CCGTCAATGTGACCTCCGATTTCGATCTGGGGGCATTCACCTACATTTTACAT 3' (c) What is the amino acid sequence of human growth hormone? (d) What is lacking from the cDNA sequence that you would ordinarily expect to find in a cDNA? (e) If you purified the fusion protein on an amylose (poly-maltose) resin and then treated the resin with Enterokinase, what are the first 5 amino acids (starting at the N terminus) of the protein released from the resin? (f) Can you think of an alternative strategy to clone exactly the same fragment of DNA into the vector in exactly the same place without using PCR? Hint: this cDNA is not very large....
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This note was uploaded on 12/20/2011 for the course BIOGD 6057 taught by Professor Fox during the Spring '09 term at Cornell University (Engineering School).
- Spring '09