2.02.10.bb - DNA duplex is not totally inflexible ABZs of...

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DNA duplex is not totally inflexible ABZs of DNA secondary structure Triple helix and G-quartets - Hoogsteen base pairing Problems: textbook Ch.8: 1, 2, 4 , 5 Study guide, p.107:#3
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The double helix is not totally inflexible 1. X-ray analysis of crystallized dodecamer (12mer) - overall structure is very much like a Watson-Crick double helix, but there are a lot of local deviations. Base sequence determines local structure 2. Neighboring bases rotate between 28° to 42° rather than a fixed 36°. 3. Opposing bases are not perfectly coplanar - assume “propellar twist. 4. Sequence can determine local structure (run of As can bend DNA)
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A DNA backbone can rotate about 6 bonds in each monomer pair 3’ end 3’ end Note: 2 strands of the helix are antiparallel
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QuickTimeª and a GIF decompressor are needed to see this picture. Sugar phosphate backbone is fexible N-glycosidc bond Propellar twists - Anti or syn conformation
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Interaction with EcoRI changes conformation of DNA Run of As bends DNA AAAAAAAA TTTTTTTT
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2.02.10.bb - DNA duplex is not totally inflexible ABZs of...

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