2.04.10.bb_1 - Physical properties of DNA and Genome...

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Physical properties of DNA and Genome Complexity Problem: Chapter 8: 8 Study guide: p. 109: 11, 1, 3 p. 117: 25-27 Final Exam will be on Thurs May 13, 7:00 - 9:30 pm
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Denaturation and renaturation of DNA Study of the forces that hold DNA duplexes together Not a chemical reaction but a physical property of DNA Do not confuse with chemical or enzymatic hydrolysis of DNA or RNA It is mostly about H-bonds Simple principle about DNA that is a recurring theme in this course: DNA can be denatured and renatured based on the complementarity of bases. The most stable form of duplex DNA is when the maximum number of H-bonds are formed.
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Forces that hold the double helix together: 1. Hydrogen bonds - most important. A=T, G=C H-bond, about 1 kCal/mole (4.4 kJ/mole) cf C-C, about 80 kCal/mole (350 kJ/mole) cooperativity gives stability 2. Stacking forces - stacked hydrophobic Pu and Py base pairs allow interactions of electron clouds, van der Waal forces, about 0.5 kCal/mole cooperativity provides stability
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G =
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2.04.10.bb_1 - Physical properties of DNA and Genome...

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