2.09.10.bb - First Prelim Thursday February 18 in class...

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First Prelim Thursday February 18 in class Material included - up to today’s lecture A - Mariano go to PLS 233 McConnell - Z go to RRB125 Reviews on Monday 4:30 - 6:00 pm Warren 245 Wednesday 5:00 - 6:30 pm Warren 131
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DNA hybridization is a measure of the relatedness between species Tm #1 70 80 90 100 Temperature (°C ) 1.4 1.2 1.0 Relative absorbance (260nm) Tm #2 a b c
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problems Genome sequencing Comparative genomics Study guide p.109:2 Restriction endonucleases see lecture notes Recombinant DNA technology (Ch.9: 1, 2, 3, 4) Read chapter 9.1 and 9.2 for the two lectures to be covered this week
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The history of genome sequencing $1/base ~1c/base Systematic versus shotgun sequencing Systematic: sequence chromosome from end to end. Align cloned segments to create a physical map before shotgun sequencing individual segments. Shotgun: Shotgun cloning of a library of sequences. Sequence all of them and then align. Segments can be short. Use computer alignment. >$10/base ~1c/10 b 2010
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Shotgun approach eliminates the time consuming step of assembling clones in a chromosome before sequencing. In this approach, random segments (600 -750 bp) of a clone (>100 kb) are sequenced and then assembled by computerized identification of overlaps. On the average, each clone has to be sequenced multiple times in order to fill all the gaps. This method was made possible because of the automation of sequencing and the development of sophisticated software. NIH: Systematic approach Celera: Shotgun approach Director of NIH Multi-billionaire (founder of Celera Genomics, Institute of Genomic Res, Craig Venter Inst.)
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Requires >10x coverage to sequence 90 % of genome. Possible only if cost of sequencing is low and computer software is sophisticated. Shotgun
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This note was uploaded on 12/20/2011 for the course BIOLOGICAL 3310 taught by Professor Goldburg during the Spring '10 term at Cornell University (Engineering School).

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2.09.10.bb - First Prelim Thursday February 18 in class...

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