3.2.10.DNA - DNA Replication is Semiconservative Meselson-Stahl experiment(1957 DNA replication begins at a specific sequence and usually proceeds

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DNA Replication is Semiconservative Meselson-Stahl experiment (1957) DNA replication begins at a specific sequence and usually proceeds bidirectionally Autoradiography Partial denaturation mapping Restriction mapping Study Guide: Topics for discussion. p.307, #2,
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E. coli chromosome contains 4.7 million bp (470,000 helical turns) which is replicated in 30 minutes. The argument against semiconservative replication is that in order for the two strands to be completely unwound, the double helix would have to be spinning at about 15,000 rpm - to many, an improbable proposition.
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(a) Conservative (b) Semiconservative (c) Dispersive After one generation After two generation Three models for DNA replication
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Cells grown in heavy medium for many generations Cells grown in light medium for the first generation Cells grown in light medium for the second generation Meselsen-Stahl Density transfer sheared Semi conservative
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Does DNA replication initiate at specific sites or at random locations? Does replication elongation proceed in one or both directions? Initiation of DNA synthesis - protein factors and enzymes involved. Chain elongation by DNA polymerases
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This note was uploaded on 12/20/2011 for the course BIOLOGICAL 3310 taught by Professor Goldburg during the Spring '10 term at Cornell University (Engineering School).

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3.2.10.DNA - DNA Replication is Semiconservative Meselson-Stahl experiment(1957 DNA replication begins at a specific sequence and usually proceeds

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