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3.4.10.the replisome - DNA Polymerases problems Ch...

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DNA Polymerases problems Ch 25:5-9, (4th ed. 5, 7-10) more in depth Study guide: Discussion topics p 308, 11-16, 21-24 The Kornberg enzyme Pol 1 QuickTimeª and a TIFF (Uncompressed) decompr are needed to see this picture Tom Kornberg Pol 2 Pol 3
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The Enzymology of DNA Replication If Watson and Crick were right, then there should be an enzyme that makes DNA copies from a DNA template In 1957, Arthur Kornberg and colleagues demonstrated the existence of a DNA dependent DNA polymerase - DNA polymerase I, also known as the Kornberg enzyme In 1959, Arthur Kornberg received the Nobel Prize in Medicine for his pioneer work on Pol1 that catalyzes “the reactions of life” Later work showed that Pol III is the principal replication enzyme in E. coli
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The three catalytic activities of DNA Polymerase I 1. 5’-3’ DNA dependent DNA polymerase activity 2. 3’-5’ exonuclease activity or proof reading function 3. 5’-3’ exonuclease activity Study guide p. 308, #7, 15-16
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The E. coli DNA polymerase I (Mr 103,000) has
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