DNA Polymerases
problems Ch 25:5-9,
(4th ed. 5, 7-10)
more in depth
Study guide: Discussion topics
p 308, 11-16, 21-24
The Kornberg enzyme
Pol 1
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Tom Kornberg
Pol 2
Pol 3
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The Enzymology of DNA Replication
•
If Watson and Crick were right, then there should be an enzyme that
makes DNA copies from a DNA template
•
In 1957, Arthur Kornberg and colleagues demonstrated the existence of
a DNA dependent DNA polymerase - DNA polymerase I, also known as
the Kornberg enzyme
•
In 1959, Arthur Kornberg received the Nobel Prize in Medicine for his
pioneer work on
Pol1
that catalyzes “the reactions of life”
•
Later work showed that
Pol III
is the principal replication enzyme in
E.
coli
The three catalytic activities of DNA Polymerase I
1.
5’-3’ DNA dependent DNA polymerase activity
2.
3’-5’ exonuclease activity or proof reading function
3.
5’-3’ exonuclease activity
Study guide p. 308, #7, 15-16
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The
E. coli
DNA polymerase I (Mr 103,000) has
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- Spring '10
- Goldburg
- DNA, DNA polymerase I, Arthur Kornberg, Tom Kornberg
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