4.6.10.RNA.bb - Replication of RNA viruses 1964: Howard...

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RNA Protein DNA Replication of RNA viruses 1964: Howard Temin noticed that DNA synthesis inhibitors prevent infection of cells in culture by RNA tumor viruses. Temin predicted that DNA is an intermediate in RNA tumor virus replication ? RT 1970: Temin and David Baltimore independently discovered the RNA-directed DNA polymerase - aka "reverse transcriptase"
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Reverse Transcriptase Primer required, but a strange one - a tRNA molecule that the virus captures from the host RT transcribes the RNA template into a complementary DNA (cDNA) to form a DNA:RNA hybrid All RNA tumor viruses contain a reverse transcriptase
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Reverse Transcriptase Three enzyme activities RNA-directed DNA polymerase RNase H activity - degrades RNA in the DNA:RNA hybrids DNA-directed DNA polymerase - which makes a DNA duplex after RNase H activity destroys the viral genome Target for HIV therapy
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Structure and functions of RNA implications for Ribozymes Transcription Transcription in Prokaryotes - some historical background Types of RNA Ch.26: 1-4 Study Guide: p326:1, 3-5,8; p.327:1
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4.6.10.RNA.bb - Replication of RNA viruses 1964: Howard...

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