4.15.10.euk transcription.bb - Final exam: May 13,...

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Unformatted text preview: Final exam: May 13, 7:00-9:30pm Uris Hall G01 Conflicts: Direct conflict or 3 exams within 24 hours Make-up exam date:TBD Prelim 3: Thur April 22 H an- O Brien Go to Riley Robb 125 The rest go to PLS 233 Transcription in Eukaryotes 1. Three RNA polymerases, I, II and III 2. Messages are monocistronic 3. Activators bind upstream at some distance from promoters 4. Positive regulation by activators . Chromatin structure represses gene expression 5. Transcription and translation uncoupled 6. Phosphorylation of transcription machinery as a means of regulation Transcription in Eukaryotes RNA polymerases I, II and III transcribe rRNA, mRNA and tRNA genes, respectively Pol I is localized in the nucleolus, a dense body in the nucleus specialized for the synthesis of ribosomal RNAs All 3 are large, multimeric protein complexes with common subunits (500-700 kD) All have 2 large subunits with sequences similar to and ' in E.coli RNA polymerase, so catalytic site is conserved Pol II is most sensitive to -amanitin, a bicyclic octapeptide from a deadly mushroom Nucleolus- location of rRNA transcription Nucleus...
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4.15.10.euk transcription.bb - Final exam: May 13,...

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