4.20.10.euk trans. regulation

4.20.10.euk trans. regulation - Prelim 3 on Thurs April 22...

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Prelim 3 on Thurs April 22 H - O go to Riley Robb 125 Everybody else go to Plant Science 233
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1. Homologous, site-specific and nonhomologous recombination 2. Eukaryote DNA replication - unique features 3. Genome-wide study of DNA replication rates, temporal order and origins. 4. Telomeres and telomerase - cloning of telomeres 5. Reverse transcriptase - no proof reading 6. Types of RNAs - Spiegelman’s experiment 7. Prokaryote and eukaryote RNA polymerases, promoters 8. Prokaryote transcription regulation - operons, positive and negative regulation. Catabolite repression (CAP=CRP). Attenuation. 9. Footprinting analysis Prelim 3 Lectures from March 16 to April 15
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Study Guide: p. 347-348 p. 351: 5 Eukaryote Gene Regulation
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Regulation of Gene Expression in Eukaryotes 1. Access to eukaryotic promoters is restricted by the structure of chromatin. 2. Every gene requires activation to be transcribed. 3. Eukaryotic cells have larger, more complex multimeric regulatory proteins than do bacteria. Combinatorial regulation by involving multiple cofactors. Eukaryote genomes are much larger. Few genes are expressed at any one time and in any one cell. The strategy for gene expression is one of positive regulation. p.166
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for known regulatory proteins. 2. Deficient in histone H1 3. Core histones (histones that form the core of nucleosomes) are more likely to be modified by acetylation or attachment of ubiquitin 4. CpG sites are undermethylated. CpG repeats are found near the 5’ upstream of many genes. Characteristics of transcriptionally active chromatin
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This note was uploaded on 12/20/2011 for the course BIOLOGICAL 3310 taught by Professor Goldburg during the Spring '10 term at Cornell.

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4.20.10.euk trans. regulation - Prelim 3 on Thurs April 22...

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