Prelim 1.key_1 - BioBM3320 Name: First Prelim Spring 2010...

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BioBM3320 First Prelim Spring 2010 Name: KEY #1 _________ #2 _________ #3 _________ #4 _________ #5 see rubrick on p.6 #6 see rubrick on p.6 #7 see rubrick on p.6 Total _________ Note: Please use pencil for graphing/drawing in case you want to make changes. Use symbols or colors to differentiate your answers on the same graph. Some of the more challenging questions do not carry many points. Do not dwell on them. Move on and go back to them later if necessary. 1
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1. (22 points) Identify and label the three bases shown below. Number the position of the atoms in one of the paired bases. Circle the pair that corresponds to the Watson and Crick base-pairing. Label the minor groove with an arc. 2 pts In this triplex structure, which pyrimidine strand is more stably associated with the structure, the Watson-Crick or the Hoogsteen base-paired strand? ___Watson-Crick _____3 pts Explain. __3-H bonds in G-C pair ______________4 pts__ Which DNA structure is more stable, G4 DNA or B-form DNA? ____G4 ____3 pts Explain. _8 H-bonds per plane in G4 DNA versus 2 or 3 H-bond per plane in B-form DNA. 4pts 2 G C C 3 7 6 3 8 9 3 1 1 1 2 2 2 4 4 4 5 5 5 6 6 minor groove
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2. (12 points) State Chargaff's rule on the base composition of DNA. _[A] = [T], [G] = [C] 4 pts Two molecules of double-stranded DNA are the same length (1,000 base pairs), but differ in base composition. Molecule 1 contains 20% A+T, molecule 2 contains 60% A+T. Which molecule has a higher Tm ? _Molecule 1 _ 4pts How many C residues are there in molecule 2? 400 ____ 4 pts 3. (12 points) Equal amounts of DNA extracted from two different species were mixed, sheared, denatured and then annealed. The melting curves of species 1 and 2 are shown below. 4 pts each
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Prelim 1.key_1 - BioBM3320 Name: First Prelim Spring 2010...

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