Prelim 3 Key - BioBM3320 Prelim 3 Spring 2010 Name: _KEY_...

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BioBM3320 Prelim 3 Spring 2010 Name: _______KEY __________________________ #1 _____ #2 _____ #3 _____ #4 _____ #5 _____ #6 _____ #7 _____ Total ______ 1
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1. (20 points) DNA recombination refers to the process when one segment of DNA is moved from one DNA molecule to another DNA molecule. Name the 3 types of recombination mechanisms. a. Homologous recombination (2 pts each) b. Site specific recombination c. Non-homologous recombination Which mechanism is involved in the repair of stalled replication forks? HR (1 pt) Name an enzyme important in this recombinational repair process DNA Polymerase 1 (1 pt) Holliday junction is an intermediate found in which of the two mechanisms? HR and SPR (1 pt) What type of recombination mechanism is used in insertion of transposons? Non-HR (1 pt) Which mechanism involves branch migration? HR In the programmed rearrangement of immunoglobulin genes, as many as 10 7 different IgG genes can be generated. What type of recombination mechanism is involved? Non-homologous recombination (1 pt) Illustrate with a diagram of the arrangement of the immunoglobulin genes in germline stem cells to show how this specialized recombination mechanism is able to generate such a diversity in antibodies. (8 points) V segments J segments C segments Stem cell Non-homologous recombination lymphocyte 2
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2. (20 points) How is the rate of DNA replication regulated in different developmental stages in eukaryotes? By regulating the number of replication origins initiated. Briefly, describe the experimental approach and the underlying principles that allow the determination of the temporal order of replication of DNA segments of a eukaryotic genome. a. Density transfer allows the separation of replicated from unreplicated DNA in a time course b. DNA microarray allows the identification of the replicated and unreplicate DNA at a genomic scale What types of information can one generate from such a study? a. Replication origins from the peaks
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Prelim 3 Key - BioBM3320 Prelim 3 Spring 2010 Name: _KEY_...

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