review1 - Wednesday Review 2.10.10 Absorption spectra for...

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Wednesday Review 2.10.10
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-As DNA is denatured, the absorbance of light at 260nm is increased -dsDNA interactions between base pairs decreases absorbance -As these interactions get disrupted, the absorbance increases - This phenomenon is called hyperchromicity Absorption spectra for Nucleic Acids
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DNA melting curve Tm Tm - A melting curve is a graph of the relative absorbance of light at 260nm vs temperature - Tm ( melting temperature) of DNA is the temperature in which half the DNA is melted - Tm depends on sequence, size, pH and ionic strength
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- As DNA is cooled it will begin to reanneal -The rate at which DNA reanneals depends on how likely two homologous strands will “run into” each other in solution -If there are more repeats in the DNA, then the greater likelihood two homologous strands will “find” each other and pair - But increasing the concentration of DNA will increase the likelihood of two matching strands finding each other. -Increasing time does this as
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This note was uploaded on 12/20/2011 for the course BIOLOGICAL 3310 taught by Professor Goldburg during the Spring '10 term at Cornell University (Engineering School).

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review1 - Wednesday Review 2.10.10 Absorption spectra for...

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