review2.24.10 - Wednesday Review 2.24.10 Polymerase Chain...

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Wednesday Review 2.24.10
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Polymerase Chain Reaction Method used to amplify DNA Start with double strand DNA Denature it single stranded DNA at high Temp Lower temperature so that primers can anneal to ssDNA Raise temp to optimize DNA polymerase, DNA elongates More time is needed for longer elongations Repeat ~30 times to amplify Serc.carlton.edu
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DNA Microarrays Way to compare gene expression in a particular condtion Ie which genes get upregulated or downregulated in response to a stress ( cancer, physical perturbation etc. ..) This requires knowing gene sequences and something about the genes neurophilosophy.wordpress.com
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Making cDNA for microarrays Isolate RNA from cells Use this mRNA to make cDNA using reverse transcriptase Can't synthesize de novo, needs a primer Can use random, specific, or ones that will prime at polyA tail Now have an RNA/DNA hybrid Digest RNA Label cDNA with two different dyes devbio.com
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review2.24.10 - Wednesday Review 2.24.10 Polymerase Chain...

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