review3.10.10 - Wednesday Review 3.10.10 Initiation of DNA...

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Wednesday Review 3.10.10
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Initiation of DNA synthesis DnaA tetramer binds to the OriC at the 9 bp repeat This causes strand separation at this location ( AT rich 13bp repeat) due to supercoiling DnaB gets delivered to location by DnaC, this binds to localized strand separation DnaB has helicase activity which further unwinds the dsDNA To prevent the strands from reanealing Single Stranded Binding (SSB) proteins come in and bind newly separated strands Primase( DnaG) primes the reaction and gyrase relieves strain
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An overview of DNA replication Now strands are separated Primase creates a short RNA primer Once the replication fork is opened, DNA pol III comes in and begins replication -tethered to DnaB Leading strand synthesis is continuous Lagging strand starts the same way, but a primase needs to prime EACH okazaki fragment, replisome dissociates and move to next Beta clamp DNA must be looped out to synthesize strands together DNA pol I removes RNA primer, ligase ligates fragments together
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review3.10.10 - Wednesday Review 3.10.10 Initiation of DNA...

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