review4.7.10 - as protein components. One of the RNA...

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Immunoglobulin genes assemble by recombination Bone marrow stem cells Each B lymphocyte has a different combination of V-J-C Programmed DNA deletion Each B lymphocyte produces an IgG that has a unique antigen binding site The light chain can combine with any of 5000 heavy chains to produce an antibody molecule 95 a.a 12 aa 4 Imprecise, variability 2.5X Bik Tye
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Mechanism of immunoglobulin gene rearrangement V(D)J Recombination RSS = recombination signal sequence R ecombination a ctivating g ene (VDJ recombinase) This reaction is reversible in a test tube, behaving like a transposition reaction.
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Telomeres- a solution to replicating linear DNA
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Extension of the 3’ end by telomerase Telomerase is an RNA dependent DNA polymerase, also known as reverse transcriptase. It is a ribonucleo- protein complex containing RNA as well
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Unformatted text preview: as protein components. One of the RNA components is a RNA template containing the sequence AACCCCAAC Greider and Blackburn, (1989) Nature, 337:331 Figure 26-35 Tetrahymena telomerase Proposed structure of telomeres - T loop EM of a T loop at the end of a chromosome isolated from a mouse hepatocyte Figure 26-35 23s 16s 5s A 260 Pulse Chase A 260 cpm Distance from the bottom Spiegelman Experiment Velocity sedimentation gradient 1. The bulk of the RNA in a cell is ribosomal RNAs and tRNAs 1. Newly synthesized RNAs are transient, heterogeneous in size and short-lived. They are mRNAs. 1. Ribsomal RNAs and tRNAs are stable. Radioactive label chased into these RNAs are not degraded. Conclusions from Spiegelmans experiment...
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review4.7.10 - as protein components. One of the RNA...

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