57 STEMS Work in pairs to prepare a bean (Phaseolus vulgaris)shoot and root (to be used later in the lab) cross section and examine the tissues found within your preparations. (Reference figures: 35.13 and 35.16 in Campbell and Reece 2005) 1. Use the bean plant from part B of this lab to obtain tissue. One partner will cut off a small section (1 - 1.5 cm) of one of the roots, the other partner will obtain a piece of stem about the same length. Stem and root segments should be taken from near the tip of the shoot or root. 2. Obtain two bolt microtomes. Microtomes are devices that are used to obtain relatively thin sections of tissues. Turn the nut of the microtome until it is almost near the tip of the bolt, forming a small depression, about 0.5 cm deep. 3. While one partner holds the bolt microtome in an upright position, the other partner should carefullypour melted paraffin into the depression. Before allowing the paraffin to completely solidify, stand the root or stem tissue on end in the paraffin.
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