IMG_0001_NEW_0037

IMG_0001_NEW_0037 - If it has a greater affinity for the...

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DEPARTMENT OF CHEMISTRY CHEM 28lL- Organic Chemistry Laboratory I Thin Layer Chromatography or TLC Thin Layer Chromatography gLC) is a powerful but very simple, quick and inexpensive analytical method used in organic chemistry for separation and identification of components in a mixture. Because of its simplicity and rapidity, it is often used to monitor the progress of a reaction and to check the purity of products. All chromatography rely on two phases: stationary and mobile. In the case of TLC, the stationary phase is a solid (silica) and the mobile phase is a liquid. This type of chromatography is done on a plate (aluminum or glass), which is coated with a thin layer of the stationary phase (silica). ln a TLC run, the sample to be analyzed partitions itself between the two phases.
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Unformatted text preview: If it has a greater affinity for the mobile phase it will move along the plate faster with the mobile phase and if it has a greater affinity for the stationary phase, it will move slower. Thus, compounds can be separated from each other based on their different polarities. In this experiment, you will use TLC to identiff a compound or a mixture of compounds in a given unknown by comparison with the reference compounds. This can be done by simultaneously running the standards with the unknown. The reference compounds are: B:2-Naphthol oo A: Naphthalene o-"*-\ lt-ltl :Y o Naphthoquinone c: SI]\,'(}I( FR,dSI]:N U\IVIIiSIIY THINKING OF 'HE WORLO...
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