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IMG_0001_NEW_0038 - I cm from the end of each...

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, DEPARTMENT OF CHEMISTRY Procedure: Obtain 2 TLC plates (one small and one 619) from the dispensary. Always hold the plates_by the J4g9!-4na do not touch the chalky side (silica). You will first run the 3 standards on the smaller plate as a practice then you are going to use the larger plate to spot the three reference compounds (A, B and c) as well as your unknown (t/) and run the TLC for identification. The TLC experiment will consist of 4 simple stages: 1. Spotting 2. Development 3. Visualization 4. Identification based on Rrvalues Spotting: Draw a light pencil line gently about
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Unformatted text preview: I cm from the end of each chromatographic plate (see diagram), but do not gouge the surface. Use micro capillaries to spot the smaller plate with the 3 stairdards, labeling them A, B, C and the other plate with four spots: the 3 standards and your unknown (A, B, C, q Please use a separate capillary for each compound. Make each spot as small as possible, preferably 0.5 mm in diameter. The less compound you spot the better and cleaner will be the visualization. 0.75-1 cm---a---+--+---a---ABCU SI\{ON I'RAS}:R U\IVnRSIIY THINKtNG OF IHE woRLn...
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