IMG_0001_NEW_0085

IMG_0001_NEW_0085 - with those of the standards and their...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
DEPARTMENT OF CHEMISTRY Visualization: Examine the plate under the UV lamp and mark the spots with a pencil. Identification based on Rr values: Develop the bigger plate using fresh solvent, mark the spots and calculate the Rr values (retention factor-sample calculation is shown below) of each spot including thaVthose of the unknown. Identiff the unknown either as a single compound or as a mixture of two compounds by comparing the unknown spoVspots
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: with those of the standards and their Rr values. Complete the report sheet and hand in it at the end of the lab. Rr calculation: The retention factor, or Rs, is defined as the distance traveled by the compound (from spotting line to center of spot) (a) divided by the distance traveled by the solvent (b). Solvent Front Spotting Line \--.-_\---' , \I\1O I'R;\SliR UNMIISIIY THINKING 0F THE w0RL0...
View Full Document

Ask a homework question - tutors are online