4 - 3. Disinfection and Sterilization Basic terms...

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Unformatted text preview: 3. Disinfection and Sterilization Basic terms sterilization disinfection Antisepsis Bacteriostasis Asepsis sterilization The process of destroying all microbial forms. A sterile object is one free of all microbial forms, including bacterial spores . disinfection The reduction or elimination of pathogenic microorganisms in or on materials, so they are no longer a health hazard. Antisepsis Use of chemical agents on skin or other living tissue to inhibit or eliminate microbes; no sporicidal ( )action is implied. Bacteriostasis Inhibits the growth of microorganisms. Asepsis No living microorganisms exists. Controlling Microorganisms By Physical Agents High Temperature Radiation Filtration Low Temperature Desiccation High Temperature static action cidal action Dry heat protein oxidation Hot air sterilization Incineration red heat flaming Moist heat denature proteins and melt lipids; more effective Autoclaving: 121 ,103 121 ,103 .4kPa .4kPa , , 20min 20min cidal for both vegetative organisms and endospores Boiling water Pasteurization : to kill particular spoilage organisms or pathogens flash method : 71.6C, 15s holding method : 62.9C, 30 min fractional sterilization : 1) Steam heating to 100 C for 30 min / Vegetative cells are destroyed but endospores survive 2) Incubate at 30 C -37 C overnight Most bacterial endospores germinate 3) Second heat treatment, 100 C, 30 min Germinated endospores are killed....
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4 - 3. Disinfection and Sterilization Basic terms...

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