15 - Mycobacterium Mycobacterium MYCOBACTERIUM...

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Mycobacterium Mycobacterium
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MYCOBACTERIUM MYCOBACTERIUM THIS GENUS IS COMPOSED OF: Strictly aerobic, acid-fast rods, does not Stain well (gram stain indeterminant), DNA has high g+c content, unique cell wall, Mycolic acid carbon chain length > c60 Relatively slow growth (two groups) A. RAPID GROWERS (Visible colonies in <5 days) B. SLOW GROWERS (Visible colonies in > 5 days) TYPE SPECIES: Mycobacterium tuberculosis
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THE GENUS MYCOBACTERIUM CAN BE DIVIDED INTO FOUR BROAD GROUPS 1. THE TUBERCULOSIS COMPLEX 2. SLOW GROWING MYCOBACTERIA OTHER THAN TUBERCULOSIS (MOTT) 3. RAPIDLY GROWING MYCOBACTERIA 4. MYCOBACTERIUM LEPRAE
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Acid Fastness Stain Acid Fastness Stain (Ziehl-Neelsen stain) (Ziehl-Neelsen stain) flood the slide with basic fuchsin (a red dye) in 5% phenol as a mordant. heat gently for few minutes to melt the wax. wash with 3% HCl in ethanol. counter-stain with methylene blue. Mycobacterium stains red and other bacteria and the background are blue. The mycolic acid and its derivatives are responsible for the acid f
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THE TUBERCULOSIS COMPLEX (Organisms that resemble M. tuberculosis; Causing a similar type of disease in humans) 1. M. tuberculosis 2. M. bovis
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Mycobacterium tuberculosis Mycobacterium tuberculosis
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M M . . tuberculosis tuberculosis General Features General Features It is a causative agent for human tuberculosis. It grows very slow with a generation time of 12-15 hours. On solid media the colonies are raised and rough with a wrinkled surface . M . tuberculosis cells grow either as discrete rods or as aggregates. Virulent strains tend to grow as an aggregated long arrangement called serpentine cord. Cord factor is a derivative of mycolic acids, trehalose 6'-dimycolate.
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Resistance Resistance : UV Malachite green(1:13000) Alcohol (to nonspore- forming bacteria) 3%HCL, 6%H 2 SO 4 , 4%NaOH (15min) Heat(62-63 ,15min) Chemical disinfectants (more) Wet Dry (highly) Sensitive Not sensitive EUGONIC GROWTH 14 DAYS DYSGONIC GROWTH 14 DAYS Mycobacterium tuberculosis Mycobacterium bovis COLONIAL MORPHOLOGY OF THE TUBERCULOSIS COMPLEX MYCOBACTERIA COLONIAL MORPHOLOGY OF THE COLONIAL MORPHOLOGY OF THE TUBERCULOSIS COMPLEX MYCOBACTERIA TUBERCULOSIS COMPLEX MYCOBACTERIA
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Transmission Transmission Through respiratory tract, alimentary tract, injured skin 。 TB in the lungs or throat can be infectious. This means that the bacteria can be spread to other people. TB in other parts of the body, such as the kidney or spine, is usually not infectious.
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Who is at risk: Who is at risk: Primary infection: children Secondary infection: age>25
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Virulence factors Virulence factors No spore , no flagellum , no exotoxin,no endotoxin, no invasive enzyme Capsule: polysaccharide;CR3;enzyme; protect Lipid/Lipo arabinomannan Heat-shock protein/Tuberculin protein: antigenicity, old tuberculin; associate with wax D can cause hypersensitivity and form tubercle
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This note was uploaded on 12/27/2011 for the course STEP 1 taught by Professor Dr.aslam during the Fall '11 term at Montgomery College.

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15 - Mycobacterium Mycobacterium MYCOBACTERIUM...

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