18-1 - Mycoplasmas Mycoplasmas A group of the smallest...

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Mycoplasmas
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Mycoplasmas A group of the smallest organisms that can be free- living in nature, Pass bacterial filter and also grow on laboratory media. More than 80 species, belong to Mycoplasmatales of Mollicute. 3 families can be divided: Mycoplasmataceae (require external cholesterol during growth, contain Mycoplasma and Ureplasma two genera); Acholeplasmataceae (need not external cholesterol during growth); Spiroplasmataceae (can form spiral structure)
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Morphology The smallest among prokaryotic microganisms with circular dsDNA, usually 0.2-0.3um in size; lack of cell wall; Pleomorphic, spherical, short rod, filament; Gram negative, but stained hardly, usually use Giemsa stain. Morphology They can assume multiple shapes including round, pear shaped and even filamentous. "fried egg" c olonial m orphology
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BIOLOGICAL FEATURES Motility: Motile by possible release and reattachment of terminal cell organelle; no flagella present; possess a protein attachment factor termed P1 that interacts with a specific cellular receptor and allows adherence to respiratory epithelium. Respiration-Fermentation: Aerobes- anaerobes.
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Culture Most aerobic; require 10%-20% human or animal serum added to basic nutrient media except Acholeplasma; typical colony show fried egg apperance . Many species are part of the normal flora
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18-1 - Mycoplasmas Mycoplasmas A group of the smallest...

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