DNA-sequencing - Sanger Technique a.k.a chain termination...

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-Narayani. N M.Sc Human Genetics
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DNA…
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So, how to read the sequence of nucleotides?? Biochemical methods Need: Basic research studying biological processes To correlate changes in gene with a diseased condition Forensics Diagnostics
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Three main methods… Maxam-Gilbert sequencing Chain termination method Dye termination method
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Maxam-Gilbert method
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Unformatted text preview: Sanger Technique a.k.a chain termination method Use of ddNTPS Dye termination method Automated DNA sequencing Applied Biosystems PRISM 377 (Gel, 34-96 lanes) Applied Biosystems PRISM 3100 (Capillary, 16 capillaries) Applied Biosystems PRISM 3700 (Capillary, 96 capillaries) Pyrosequencing...
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DNA-sequencing - Sanger Technique a.k.a chain termination...

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