molecularvirology

molecularvirology - OverviewofMolecularEpidemiological

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
    Overview of Molecular Epidemiological  Methods for the Subtyping and  Comparison of Viruses Derek Wong http://virology-online.com
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Uses of molecular  epidemiological methods Subtyping - in some viruses, different subtypes are associated with different clinical manifestations e.g. enteroviruses, adenoviruses, and human papillomaviruses. General Epidemiology - by identifying the viral subtypes at different times and geographical locations, one can detect major changes in the epidemiological patterns of infection e.g. HIV and HCV. Investigation of Outbreaks - to support or disprove a link between the donor and recipient viruses e.g. HIV, HBV, HCV, Norwalk virus.
Background image of page 2
Methods Used - Complete or  Partial genome? For greatest degree of accuracy, the complete genome should be used for the purpose of comparison. However, since viral genomes ranges from 3500 bp to over 200,000 bp, it would be highly impractical to sequence the whole genome. Certain simple methods are still used for the comparison of complete genomes e.g. RFLP for CMV, HSV, and Adenoviruses. Nowadays in practice, a small part of the genome is amplified first by PCR and the product investigated by sequencing or other methods.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Strategies for identification of the PCR  Product (Commonly used methods) Sequencing of the PCR product the gold standard but expensive and not widely available. PCR product may be sequenced directly or cloned before sequencing.
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 11

molecularvirology - OverviewofMolecularEpidemiological

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online