pcr - PCR AND ITS USES IN DIAGNOSIS OF INFECTIOUS DISEASES

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PCR AND IT’S USES IN DIAGNOSIS OF INFECTIOUS DISEASES
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A simple rapid, sensitive and versatile  in vitro   method  for  selectively  amplifying  defined  sequences/regions of DNA/RNA  Requires  the  binding  of  short  sequences  of  DNA( oligonucleotides/amplimers/primers to  complementary  sequences  flanking  the  desired  target region The  action  of  an  enzyme  (DNA  polymerase)  to  synthesise new exact copies of the target DNA
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RESULTS
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PCR useful in diagnosis of various infectious diseases Can rapidly and accurately detect presence of Chlamydia, mycoplasms, mycobactria, herpes viruses and entero viruses Is also used to quantify viral load and detect antimicrobial resistance
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Reverse transcription PCR is also used initial template is RNA rather than DNA enzyme reverse transcriptase first converts the RNA target into a complementary DNA copy (cDNA)
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Diagnosis of infectious diseases
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earliest commercial tests -PCR assay for the 
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This note was uploaded on 12/27/2011 for the course STEP 1 taught by Professor Dr.aslam during the Fall '11 term at Montgomery College.

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pcr - PCR AND ITS USES IN DIAGNOSIS OF INFECTIOUS DISEASES

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