Ch03 - 8/30/2011 Chapter3MicrobialCellStructureandFunction...

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8/30/2011 1 Chapter 3 Microbial Cell Structure and Function Borrelia sp. European cause of Lyme Disease Cell membrane purple Cell Wall green Internal Flagella – yellow Sheath blue Bacterial Shapes
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8/30/2011 2 Bacterial Sizes Epulopscium fischelsoni and Paramecium E. fischelsoni is 75 μ m wide and 600 μ m long
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8/30/2011 3 Thiomargarita namibiensis These bacteria are 400 μ m in diameter Cell Sizes
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8/30/2011 4 Surface Volume Relationship Lets Get Into These Cells The most used models: Escherichia coli and Bacillus subtilis
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8/30/2011 5 Major Parts and Molecular Players
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8/30/2011 6 2 D Electrophoresis Spreads out the Proteins Differential Centrifugation to Isolate Cell Parts Ribosomes with some m RNA
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8/30/2011 7 X ray diffraction Crystallographic Analysis Reveals Structure X ray diffraction crystallography shows the three dimensional form of cell components at th ti l the atomic level. Helps relate structure to function Mutant strains are selected for loss of a given function, or a strain can be intentionally mutated so as to lose or Genetic Analysis Reveals Function alter a gene. Figure 1.1 Figure 3.6
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8/30/2011 8 Cell Envelope Cell Membrane – Phospholipid bilayer Phopholipid + Proteins Cell Wall–Pept idog lycan +/ Outer Membrane Gram Positive Gram Negative Plus Other Stuff: flagella, pili, capsules/slime Phospholipid Membrane
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8/30/2011 9 Fluid Mosaic Model
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8/30/2011 10 The Major Phospholipid Other Phospholipids
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8/30/2011 11 The Fatty Acids Hopanoids Stabilize Membrane Structure
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8/30/2011 12 Sterols and Hopanoids Sterols, Hopanoids and Branched Chain lipids Share Common Synthetic Pathway Archaean Lipids
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8/30/2011 13 Comparison of Membrane Lipids Major Membrane Functions
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8/30/2011 14 Bacterial Cell Membrane Functions 1. Permeability Barrier 2. Transport 3. Respiration and Photosynthesis 4. Cell Membrane Synthesis 5. Cell Wall Synthesis 6. Motility – Flagella Insertion and Motor 7. Information Processing–Env ironmenta l Sensing 8 Attachment of Chromosome (DNA) 8.
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Ch03 - 8/30/2011 Chapter3MicrobialCellStructureandFunction...

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