Nanotubes - Frequency of plasmid transfer was 10 ‐ 4 to...

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3/3/2011 1 Breakthrough: Bacterial Nanotube Communication Bacterial Nanotubes Dubey, G.P. and S. Ben Yehuda. 2011. Intercellular nanotubes mediate bacterial communication. Cell 144 :590 600
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3/3/2011 2 Transfer of GFP A. Mix of B. subtilis PY79(gfp ) and SB444 (gfp + ) B. Fluorescence in gfp cells as a function of distance from gfp + cells C. a. 0 min; b. 30 min; c. 60 min. D. Fluorescence of gfp cells as a function of distance from gfp + cells. SEM: The Nanotubes Nanotubes are obvious: arrows Are they originally tiny: B dashed line area. E. F. G. Gold conjugated anti gfp Ab approach or are in nanotubes.
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3/3/2011 3 Look at These Tubes! Nanotubes formed between: B. subtilis and S. aureus B. subtilis and E. coli E coli and S aureus E. coli S. aureus Transient Non hereditary Phenotype Transfer
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3/3/2011 4 Plasmids Transfer in Nanotubes Nonhereditary Hereditary
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Unformatted text preview: Frequency of plasmid transfer was 10 ‐ 4 to 10 ‐ 7 Nature of Nanotubes: Effect of SDS 1. SDS at 0.009% allows B. subtilis to grow (A. . LB). 2. SDS at 0.009% stops nonhereditary transfer (P1+P2…Cm+Kan). 3. SDS does not increase Cm or Kan sensitivity: serial dilutions 3 ‐ 6 to 3 ‐ 10 (B) 4. SEM: no ‐ nanotubes in 0 007% SDA (C ) but 0.007% SDA (C ) but nanotubes lacking SDS (D). Thus nanotube transfer is SDS sensitive. 3/3/2011 5 Bacterial Nanotubes Transfer of CARGO or Signals or Both? What is the chemical structure of the tube ? How does the membrane fit in (get through wall peptidoglycan and into the tube) ? What are the energetics ? Who drives what into whom ? What is the relationship to the cytoskeleton ?...
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