Study Questions 20-23

Study Questions 20-23 - Study Questions 20-23 1 W hat are...

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Study Questions 20-23 1. What are the major differences between prokaryotic and eukaryotic transcription? 2. Which hypothesis regarding eukaryotic RNAPs was proven with -amanitin and actinomycin D (be specific)? 3. Which genes are transcribed by RNAP I? RNAP II? RNAP III? 4. What does CTD stand for? Explain the role of CTD tail in eukaryotic gene expression? 5. How would you define enhancers? What are their characteristics? What is the difference(s) between enhancer and upstream control element? 6. Explain the use of reporter genes for estimation of promoter strength. 7. Explain briefly 5’ deletion series. What kind of information do they reveal? 8. Explain the modular nature of RNAP II promoters. 9. Draw a diagram of RNAP II promoter (show all types of elements that we have mentioned in class). 10. Explain the tissue (cell type) specificity of eukaryotic cis elements. 11. Knowing that different genes may have the same promoter and enhancer elements and that different transcription factors contain the same structural features, how would you explain
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Study Questions 20-23 - Study Questions 20-23 1 W hat are...

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