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19 - Transcription (Prokaryotes); Regulation of the Operons (2)

19 - Transcription (Prokaryotes); Regulation of the Operons (2)

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Plasmid can carry I gene as well…or only I gene (and not operon) Different combinations….. Also combination of these mutation with mutations in structural genes (as products of structural genes ARE actually measured) J & M: Proof through complementation tests Transformation of the bacterial cells with plasmids carrying lac operon (whole or parts) - result is partially diploid cell MERODIPLOID Purpose: artificially produced 2 sets of “alleles” for lac operon genes, complementation is enabled. Discovered/defined cis-elements and trans factors
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