Plant Anatomy An Applied Approach (Lecture ppt) Ch10_PracticalMicrotechnique

Plant Anatomy An Applied Approach (Lecture ppt) Ch10_PracticalMicrotechnique

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Ch.10 Ch.10 Practical microtechnique Practical Gwo­Ing Liao Department of Life Sciences, National Cheng Kung University Safety considerations Safety Health & Safety Wear appropriate protective Clothing Glove Goggle Disposable active carbon face mask p.170 p.170 ry_glove_vinyl.jpg Safety considerations Safety p.170 Appropriate use of fume cupboard At least two persons Waste disposal 國國國國國國國國國國國 e/P1010350.jpg 國國國國國國國國國國國國國國國國國 國 國 國 國 國 國 國 國 國 國 國 國 國 國 (GHS)- 國 國國國國國國國國國國國國國國國國國國國國 國國國國 國國國國國國國國國國國國國 國 國國國國國國國國 國 國 GHS 國 國 國 國 p.170 Materials Materials “Standard” plant → study “Standard plant list” → teaching Selected study material (Appendix 1, p.195) (Appendix Anatomical characters Families Local floras Anatomical leaf, stem, a few examples (p.195~200) (p.195~200) softwood, hardwood (p.201~202) (p.201~202) Materials Materials p.170 Typical & Atypical Zea maize ←→ monocotyledon Typical plant- morphology, physiology … Zea maize, barley, rice…… peas, lettuce, sunflower, tobacco, beet, tomato, potato, Arabidopsis thaliana…… Materials- Anatomical studies Materials- p.171 Fresh material & Dried herbarium material Cell structure and histology - better to fix Fixative - killing and preserving cell contents Kill the protoplast without distortion Preserve to fix the fine detail Harden the material Methods- Fixatives Methods- p.171~173 Fixatives Alcohol and formaldehyde-based fixatives FAA, FPA→ wax wax formalin 國 國 國 -acetic acid - alcohol國 Table formalin acetic alcohol Table 10.1 國 formalin -propionic acid -alcohol 10.1 Non-coagulating fixatives osmium tetroxide 國 國 國 國 , acrolein 國 國 國 tetroxide acrolein glutaraldehyde 國 國 國 , formaldehyde→ plastics Methods- Dehydration Dehydration → Infiltration p.173~175 Dehydration→ Infiltration Alcohol series→ xylene 國 國 國 → xylene wax wax TBA series→ wax 國 Table 10.2 國 wax • TBA 25.5℃ • Wax 50~70℃ 國 48hrs Methods- Sectioning Methods- p.175 Sectioning- magnifications of 100~400x Freehand sections Rotary microtome wax-embedded 10μm specimens thick Methods- Sectioning Methods- p.175~179 Sliding microtome 15~30μm thick Wood 國 Fig. 10.1 國 Twigs 國 Fig. 10.2~3 國 Leaves 國 Fig. 10.4 國 silica bodies- 10% hydrofluoric acid 50% alcohol Methods- Clearing MethodsClearing 50% alcohol ↓ water ↓ sodium hypochlorite p.177~179 Methods- Staining Methods- p.179~183 Staining temporary & permanent Temporary stains Double stain FABIL Safranin and haematoxylin Safranin and fast green 11.Basic histochemistry p.237, CD-ROM Factfile index Methods Methods Preparing permanent slides Freehand sections 國 Table 10.3 國 Paraffin sections p.183~187 Methods Methods Preparation of surfaces Leaf surfaces 國 Fig. 10.6 國 Stem surfaces Surface replicas Cuticular preparations Clearing material p.189~191 Methods Methods Standard levels 國 Fig. 10.7 國 Leaf Petioples Stems Roots p.191 Microscopy Microscopy p.191~194 LM light microscopy (OM optical microscopy) TEM transmission electron microscopy SM stereo microscopy SEM scanning electron microscopy Microscopy and the interpretation…… Appendices- methods in microscopy p.205, CD-ROM The virtual plant ...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online