Enzymes - Enzymes (If you dont have the energy, we can...

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Enzymes (If you don’t have the energy, we can help!)
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Enzymes Biological catalysts Made primarily from proteins Bind to substrate Not used up Speed up reaction
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Catalysis Uncatalyzed G time R P Transition E A time R P G Catalyzed
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Catalysts Increase rate of reaction Provide activation energy Energy is conserved Activation energy has to come from somewhere
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Sources of Activation energy Binding energy Solvent released from active site when substrate binds increases ΔS surr Induced fit Enzyme forces substrate into unstable transition state Catalytic antibodies Binding substrate brings reactive groups together
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Catalysis by an enzyme E + S > ES > EP > E + P
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Enzyme Example Serine Proteases http://www.bio.cmu.edu/courses/03231/Protease/Ser Active site and substrate Note side chain interactions Substrate diffuses into active site
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Serine proteases Substrate binds to active site Chemical groups interact Force substrate into unstable intermediate
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Serine Proteases Peptide bond is cleaved Serine in active site is bound to carboxyl side of peptide
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Serine proteases Half of protein diffuses out Enzyme used up Has to be regenerated
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Serine proteases Water diffuses into active site Juxtaposes chemical groups Similar reaction to first
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Serine proteases New unstable intermediate generated
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Serine Proteases Carboxyl end of peptide diffuses out Enzyme regenerated Ready for another round
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This note was uploaded on 12/28/2011 for the course STEP 1 taught by Professor Dr.aslam during the Fall '11 term at Montgomery College.

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Enzymes - Enzymes (If you dont have the energy, we can...

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