profunction - Protein Function Structure will determine the...

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Protein Function Structure will determine the function of the protein
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Key ideas and terms protein can bind a ligand in the binding site For an enzyme, the ligand is a substrate and they bind in what is called the active site ligand has to be the correct shape ligand has to have the complementary charges and hydrophobicity or hydrophilicity
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Lock and Key Hypothesis Protein and ligand have complementary shapes. Interactions must also be complementary If enzyme charge is negative, substrate must be positive If pocket is nonpolar, ligand must be nonpolar Antibodies
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Induced Fit Induced Fit: when the protein and ligand bind, the protein may change conformation to allow for tighter binding Frequently, both the ligand and the protein change conformation
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Examples: O 2 binding proteins: myoglobin and hemoglobin oxygen is not water soluble yet needs to be transported diffusion is not effective myoglobin is found primarily in muscle tissue Hemoglobin is in the blood Both proteins contain heme
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Heme Group consists of a Fe 2+ and a protoporphyrin ring to help stabilize the iron(II) ion
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Heme Group the iron must be a 2+ to bind oxygen. The heme group is buried deep within the protein so that the iron is not oxidized to 3+ there must be flexibility in the protein to allow for oxygen to attach and then let go Iron has 6 coordination sites. Four of them used by porphyrin. Unshared pairs on nitrogen complex to iron Fifth and 6 th for oxygen and protein Heme is planar
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Myoglobin has heme group eight alpha helical segments dense hydrophobic core all but two polar groups on outside room for only 4 water molecules flat heme in pocket iron coordinated to poryphorin and H As well as the heme
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Myoglobin Binding Curve Hyperbolic binding curve Relatively insensitive to small changes in oxygen concentration
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This note was uploaded on 12/28/2011 for the course STEP 1 taught by Professor Dr.aslam during the Fall '11 term at Montgomery College.

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profunction - Protein Function Structure will determine the...

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