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DNATechnology160-page3 - bacterium dies its plasmids are...

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Biotechnology - 3 Much of the emphasis of this chapter in your text is to provide some information about the applications of recombinant DNA research, and in biotechnology in general, as well as some of its techniques. We shall look at some of those processes briefly. Natural DNA Recombination in Prokaryotes Bacteria are particularly useful for research in biotechnology. Recombination in bacteria is common. Transformation, first discovered by Griffith, occurs naturally in many bacteria, and is a good example of recombination. Bacteria also have recombination using plasmids, small independent pieces of DNA incorporated into bacteria directly from the environment. A bacterium may have multiple copies of plasmids, or clones, and when the
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Unformatted text preview: bacterium dies, its plasmids are released into the environment where they can be incorporated into a different bacterium. Since plasmids carry independent genes, new information is incorporated into the DNA molecule. DNA technology uses modified plasmids to add desired genes from plasmid clones to host cells for research purposes, and for the manufacture of chemicals needed by humans. Often plasmids that have antibiotic resistance, called R plasmids, are chosen, because they can also be used to determine if the desired gene has been incorporated into the host bacterium. The modified plasmids that carry DNA to the target host are cloning vectors....
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