DNATechnology160-page8

DNATechnology160-page8 - Reaction (PCR). PCR is very...

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Biotechnology - 8 Gel electrophoresis separates charged molecules based on their molecular weight. An electric current is used to "drive" molecules that are placed in wells made in the gel from the negative electrode of the gel chamber toward the positive electrode. The rate at which molecules move through the gel is relative to their molecular weight. As the molecules are separated they appear as distinct bands on the gel. DNA fragments have a strong negative charge in neutral pH so they are well suited for the technique of gel electrophoresis. Obtaining A Sufficient Amount of the Target DNA Source – PCR Once a gene has been located, researchers obtain multiple copies of the gene for their work. One method to obtain sufficient DNA is the Polymerase Chain
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Unformatted text preview: Reaction (PCR). PCR is very valuable when trying to do a detailed analysis of a DNA molecule. PCR is also valuable when there is just a tiny amount of DNA from which to start. This is often the case when one is using DNA materials for potential evidence in criminal investigations, or when one is trying to reconstruct DNA from preserved and fossil materials. Kary Mullis won the Nobel prize for his 1986 development of PCR. PCR uses alternating heating and cooling cycles starting with heated single-stranded DNA, primers that can join complementary DNA and DNA polymerase isolated from thermophilic bacteria to synthesize new molecules. Polymerase Chain Reaction...
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