DNATechnology160-page9 - Once a genome map is available...

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Biotechnology - 9 Applications of Biotechnology Genome Mapping The techniques of recombinant DNA technology have been used to generate the gene sequences of species, or the species entire genome. This has been for several different organisms, including the human genome. The same techniques can also be used to compare genes from different individuals and from different species. The human genome project, on-going for more than a decade, was deemed completed in summer, 2000. Genome projects include gene mapping using recombination frequencies and DNA restriction fragment mapping, restriction enzymes and DNA probes repeatedly along a chromosome with known markers, along with some special sequencing techniques.
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Unformatted text preview: Once a genome map is available, genes are studied to identify nucleotide variations for gene disorders, allele differences, and to study the relationship of genes to gene expression. Genomics has been very useful for genetic diseases such as cystic fibrosis and sickle cell anemia. We also use our knowledge of the genome to look for new gene sequences in different organisms. For example, the homeotic genes that affect developmental sequences in all animals can be isolated from a well-known species, such as the fruit fly, and then used as a DNA probe to see if a comparable gene is also in other animals, including humans. DNA Sequencing...
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