IMG_0004_NEW_0007_001 - tfNl u t s,tlut u tlllvlutH In...

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.:,:.,j:*11l,flffireryry stBB 22210-3 IxsrnucroR: Dr. Tarailo-Graovac 7 MIDTERM EXAVI II July 12fr,2010 Which of the following is a firnction of DNA polymerase I in DNA replication in E. coli? 4) 3'-5' chain extension of Okazaki fragments (O)S'-l' excision (editing) to remove the RNA primer nucleotides Y5'-3' editing (proof-reading) to remove mismatched nucleotides @ att of the above A tautomeric shift causing the substitution of one purine for another purine is called: a) translocation b) transversion c) deletion @ransition 17) Which of the following chemical mutagens are likely to cause base pair insertions? h a) 5-bromouracil 1q) b) Nitrous acid ftOe.riaine orange @Oo-*.thylguanine Xhich of the following enzymes repairs DNA damage using "direct repair methods"? L{DNA glycosylases -b\uvrABC i..jbu-ur ryl guanine alkyltransfe ras e
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Unformatted text preview: tfNl u t s,tlut u tlllvlutH In bacteria, which enzyme unwinds double-stranded DNA until it meets a Chi site? a) uvrABC /@-ecBCD c) RecA d) Cre recombinase 20) -Itre sites at which a restriction endonuclease cuts the DNA molecule are known as: ts [ u1]. .co-enition sequences \sticky sites c) Blunt ends @ nestriction maps The polymerase chain reaction (PCR) is based on the repetition of cycies of DNA replication by a thermostable D\A polymerase. These steps are DNA replication, primer annealing, and template denaturation. In rvhat order should these steps be taken to achieve the desired results? a) Replication, annealing, denaturation b) Replication, denaturation, annealing Q Annealing, denaturation, replication /a) l"ttututation, annealing, replic ation {,/ ,/...
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This note was uploaded on 12/29/2011 for the course MBB 222 taught by Professor Briscoe during the Fall '10 term at Simon Fraser.

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