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IMG_0004_NEW_0057 - in the vector The transgene is present...

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8) A linear fragment of DNA is cleaved with the individual restriction enzymes HindIII and Smal and then with a combination of the two enzymes. The fragments obtfired are: Hindrrr z'kb+s.'kb fu)7n1{'trattu' {, Ak-{ 3' smal 2.0kb+ s.skb @D,ykL 3' -rt Lq4A s', Hindlrr + smar 25kb + 3.0W2 .oo, ,-tllr, , r, c c cB (tL> 3 / a) Draw the restriction map. d OCtC)Cfc f , (tra;t-) i '.5*t) q,r.RT b) The mixture of fragments produced by the combined enzymes is cleaved with the enzyme EcoRI. resulting in the loss of the3kb fragment and the appearance o&_!3,nq stained with ethidi t. Mark the EcoRI tt aln 6tln J.J APRa qW + zs.Yb sth t^Rl- + SN'2 4W+ 1.5?! + z?b tw 9) a) Outline a procedure to insert a
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Unformatted text preview: in the vector. The transgene is present in a fragment that was digested using Psil. (Refer to lecture notes for pBR322 structure.) eg, rJ t.s r >) dtffi*, Ttvryytau . 4r*yd,"U"), * Tef,/arff\ b) Briefly describe several rationales for constructing and analyzingtransgenic organisms. 01, *A ovvry\sy1 / ryr,rb"W ?F +/MW tu a. ,"-t'^il ;r,l[,"lt'r 6rn6. O 1o *^t sk ,W 1 ,,''^+^+e"A '*W in etr y /ry*"'r, g {, w l*'bf, y,* nfut'*rtt # tufl We) J J* /",'.rkovE' ?,Il'b [email protected]'. t' cfrn rrc:' 4 c rT Adbs' a 0 X, frvWfuw-1*,^",^'rl{0 " 1'vt'ca ifu W rtr o il'"qh g 7, & ,^'l' P^h {tu 1a*'&,,t 1 int'i{...
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